Glucagon ELISA - 10 µL(10-1281-01)

Kit format: 1 x 96 wells

Measurement range: 2-180 pmol/L (7 - 627 pg/mL)

• Low sample volume - 10 µL
• No purification of samples
• Sample types: Serum, EDTA Plasma, and cell-culture medium
• Measurement range: 2 - 180 pmol/L (7 - 627 pg/mL)
• Detection limit: 1.5 pmol/L (5.23 pg/mL)
• Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
• Highly appreciated and often cited assay

Mercodia Glucagon ELISA - 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.

A revolutionizing ELISA for sensitive and specific determination of glucagon in rat, mouse, and non-human primate (NHP) samples. A research tool advancement that allows science to take the next step.

Mercodia Glucagon ELISA – 10 µl was launched in 2015 and has since contributed to advancing research by allowing highly sensitive measurements of glucagon. The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere. A sequential protocol has been developed to eliminate this interference, helping to achieve the highest possible specificity (see Technical Note TN34-162 “Alternative sequential protocol”).

Test characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 µL
Assay range: 2-180 pmol/L (7 - 627 pg/mL)
Detection limit: 1.5 pmol/L
Incubation: 18-22 h (overnight) + 15 min
Detection method: absorbance

Specificity

Mini-glucagon, n.d.
Glicentin, human 1.0 %
Glicentin, mouse 7.0 %
Glicentin, rat 4.0 %
Oxyntomodulin, human/rat/mouse 2.0 %
Oxyntomodulin, bovine/canine/porcine n.d.
GLP-1 (7-36), n.d.
GLP-1 (9-36), n.d.
GLP-2, n.d.

n.d.= not detected