Glicentin ELISA(10-1273-01)

Kit format: 1 x 96 wells

Measurement range: 3 – 300 pmol/L (24 – 2400 pg/mL)

• Highly specific, sensitive and rapid method for determination of glicentin
• No cross-reactivity to circulating proglucagon derived gut peptides
• Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin

The Mercodia Glicentin ELISA is a high quality immunoassay for the quantification of human glicentin in serum, plasma and cell culture media.
 

Glicentin is a proglucagon derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in the circulation after stimulation and is often a problem when measuring glucagon and other proglucagon derivates because of it's shared sequence. The Mercodia Glicentin ELISA however, has no cross-reactivity to such circulating proglucagon derived gut peptides.

Test characteristics

Samples: Serum, EDTA plasma and cell culture media
Sample volume: 25 µL
Assay range: 3 - 300 pmol/L (24-2400 pg/mL)
Detection limit: 3 pmol/L (24 pg/mL)
Incubation: 1 h + 1 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Glucagon N.D.
Oxyntomodulin N.D.
Mini-glucagon N.D.
GLP-1 N.D
GLP-2 N.D.
Rat glicentin 0.9 %
Mouse glicentin N.D

N.D = Not detected